{"id":15834,"date":"2024-05-04T16:19:07","date_gmt":"2024-05-04T13:19:07","guid":{"rendered":"https:\/\/cmcpal.com\/site\/?post_type=product&p=15834"},"modified":"2024-05-04T16:21:10","modified_gmt":"2024-05-04T13:21:10","slug":"sperm-antibody-elisa-ref-eia-1826","status":"publish","type":"product","link":"https:\/\/cmcpal.com\/site\/product\/sperm-antibody-elisa-ref-eia-1826\/","title":{"rendered":"Sperm Antibody ELISA \/ REF: EIA-1826"},"content":{"rendered":"
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Sperm Antibody<\/strong><\/h2>\n
\nAn enzyme immunoassay for the quantitative determination of antibodies directed against spermatozoa antigens in serum. The Sperm Antibody ELISA (Enzyme Linked ImmunoSorbent Assay) is a solid-phase sandwich enzyme-immunoassay for the quantitative determination of anti-spermatozoa antibodies in human serum. The ELISA-plate is coated with a mix of spermatozoa proteins which are recognized by anti-spermatozoa antibodies. The samples and standards are pipetted into the wells and then incubated. During this incubation anti-spermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. After washing the enzyme conjugate, consisting of anti-human globulin antibodies covalently coupled to horseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3\u2019,5,5\u2019-tetramethylbenzidine) yielding a color reaction which is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength ?550 nm is recommended.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

Sperm Antibody An enzyme immunoassay for the quantitative determination of antibodies directed against spermatozoa antigens in serum. The Sperm Antibody ELISA (Enzyme Linked ImmunoSorbent Assay) is a solid-phase sandwich enzyme-immunoassay for the quantitative determination of anti-spermatozoa antibodies in human serum. The ELISA-plate is coated with a mix of spermatozoa proteins which are recognized by anti-spermatozoa…<\/p>\n","protected":false},"featured_media":15837,"comment_status":"open","ping_status":"closed","template":"","meta":[],"pwb-brand":[167],"product_brand":[],"product_cat":[135,146,111,118],"product_tag":[],"class_list":{"0":"post-15834","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"pwb-brand-drg","7":"product_cat-drg","8":"product_cat-elisa-drg","9":"product_cat-laboratory","10":"product_cat-reagents","11":"description-off","13":"first","14":"instock","15":"shipping-taxable","16":"product-type-simple"},"brands":[{"id":167,"name":"DRG","slug":"drg"}],"_links":{"self":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product\/15834","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/comments?post=15834"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/media\/15837"}],"wp:attachment":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/media?parent=15834"}],"wp:term":[{"taxonomy":"pwb-brand","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/pwb-brand?post=15834"},{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product_brand?post=15834"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product_cat?post=15834"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product_tag?post=15834"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}