{"id":15807,"date":"2024-05-04T16:06:45","date_gmt":"2024-05-04T13:06:45","guid":{"rendered":"https:\/\/cmcpal.com\/site\/?post_type=product&p=15807"},"modified":"2025-06-25T14:39:44","modified_gmt":"2025-06-25T11:39:44","slug":"ebv-vca-igm-elisa-ref-eia-3476","status":"publish","type":"product","link":"https:\/\/cmcpal.com\/site\/product\/ebv-vca-igm-elisa-ref-eia-3476\/","title":{"rendered":"EBV-VCA IgM ELISA \/ REF: EIA-3476"},"content":{"rendered":"
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EBV-VCA IgM\u00a0<\/strong><\/h2>\n
An enzyme immunoassay for the qualitative and semiquantitative determination of IgM-class antibodies to Epstein-Barr viral capsid antigen in human serum. The DRG Epstein-Barr Virus (VCA) IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results. Microtiter wells as a solid phase are coated with inactivated Epstein-Barr viral capsid antigen gp 125. Pretreated patient specimens and ready-for-use controls are pipetted into these wells. During incubation Epstein-Barr viral capsid antigen-specific antibodies of positive specimens and controls are bound to the immobilized antigens. After a washing step to remove unbound sample and control material horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells. During a second incubation this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes. After a second washing step to remove unbound conjugate the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color. The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid. The intensity of this color is directly proportional to the amount of Epstein-Barr viral capsid antigen-specific IgM antibody in the patient specimen.\u00a0 Absorbance at 450 nm is read using an ELISA microtiter plate reader.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

EBV-VCA IgM\u00a0 An enzyme immunoassay for the qualitative and semiquantitative determination of IgM-class antibodies to Epstein-Barr viral capsid antigen in human serum. The DRG Epstein-Barr Virus (VCA) IgM ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA). Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper-immune anti-human IgG-class antibody…<\/p>\n","protected":false},"featured_media":109801,"comment_status":"open","ping_status":"closed","template":"","meta":[],"pwb-brand":[167],"product_brand":[],"product_cat":[135,146,111,118],"product_tag":[],"class_list":{"0":"post-15807","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"pwb-brand-drg","7":"product_cat-drg","8":"product_cat-elisa-drg","9":"product_cat-laboratory","10":"product_cat-reagents","11":"description-off","13":"first","14":"instock","15":"shipping-taxable","16":"product-type-simple"},"brands":[{"id":167,"name":"DRG","slug":"drg"}],"_links":{"self":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product\/15807","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/comments?post=15807"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/media\/109801"}],"wp:attachment":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/media?parent=15807"}],"wp:term":[{"taxonomy":"pwb-brand","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/pwb-brand?post=15807"},{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product_brand?post=15807"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product_cat?post=15807"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product_tag?post=15807"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}