{"id":15777,"date":"2024-05-04T15:51:45","date_gmt":"2024-05-04T12:51:45","guid":{"rendered":"https:\/\/cmcpal.com\/site\/?post_type=product&p=15777"},"modified":"2024-05-04T15:52:43","modified_gmt":"2024-05-04T12:52:43","slug":"vzv-igm-elisa-ref-eia-3524","status":"publish","type":"product","link":"https:\/\/cmcpal.com\/site\/product\/vzv-igm-elisa-ref-eia-3524\/","title":{"rendered":"VZV IgM ELISA \/ REF: EIA-3524"},"content":{"rendered":"
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VZV IgM\u00a0<\/strong><\/h2>\n
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The DRG VZV IgM ELISA RUO is an enzyme immunoassay for the qualitative and semiquantitative determination of IgM -class antibodies to Varicella zoster Virus in human serum or plasma (EDTA, heparin or citrate plasma).\u00a0 This product is for Research Use Only; International customers contact your representative to receive information about the CE marked version.<\/div>\n
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The DRG VZV IgM ELISA is a solid phase enzyme-linked immunosorbent assay (ELISA).\u00a0 Patient samples are diluted with Sample Diluent and additionally incubated with IgG-RF-Sorbent, containing hyper immune anti-human IgG -class antibody to eliminate competitive inhibition from specific IgG and to remove rheumatoid factors. This pretreatment avoids false negative or false positive results.<\/div>\n
Microtiter wells, as a solid phase, are coated with inactivated Varicella zoster Virus antigen (wild strain isolate). Diluted patient specimens and ready-for-use controls are pipetted into these wells. During incubation Varicella zoster Virus- specific antibodies of positive specimens and controls are bound to the immobilized antigens.<\/div>\n
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After a washing step to remove unbound sample and control material, horseradish peroxidase conjugated anti-human IgM antibodies are dispensed into the wells.\u00a0 During a second incubation, this anti-IgM conjugate binds specifically to IgM antibodies resulting in the formation of enzyme-linked immune complexes.<\/div>\n
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After a second washing step to remove unbound conjugate, the immune complexes formed (in case of positive results) are detected by incubation with TMB substrate and development of a blue color.\u00a0 The blue color turns into yellow by stopping the enzymatic indicator reaction with sulfuric acid.\u00a0 The intensity of this color is directly proportional to the amount of Varicella zoster Virus-specific IgM antibody in the patient specimen.\u00a0 Absorbance at 450 nm is read using an ELISA microtiter plate reader.<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

VZV IgM\u00a0 The DRG VZV IgM ELISA RUO is an enzyme immunoassay for the qualitative and semiquantitative determination of IgM -class antibodies to Varicella zoster Virus in human serum or plasma (EDTA, heparin or citrate plasma).\u00a0 This product is for Research Use Only; International customers contact your representative to receive information about the CE marked…<\/p>\n","protected":false},"featured_media":15778,"comment_status":"open","ping_status":"closed","template":"","meta":[],"pwb-brand":[167],"product_brand":[],"product_cat":[135,146,111,118],"product_tag":[],"class_list":{"0":"post-15777","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"pwb-brand-drg","7":"product_cat-drg","8":"product_cat-elisa-drg","9":"product_cat-laboratory","10":"product_cat-reagents","11":"description-off","13":"first","14":"instock","15":"shipping-taxable","16":"product-type-simple"},"brands":[{"id":167,"name":"DRG","slug":"drg"}],"_links":{"self":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product\/15777","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/comments?post=15777"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/media\/15778"}],"wp:attachment":[{"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/media?parent=15777"}],"wp:term":[{"taxonomy":"pwb-brand","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/pwb-brand?post=15777"},{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product_brand?post=15777"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product_cat?post=15777"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/cmcpal.com\/site\/wp-json\/wp\/v2\/product_tag?post=15777"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}